A 1 kb insert (i) is ligated to 5 kb vector (v)in molar ratio of i:v of 5:iin a reaction volume of 1 ml at final dna concentration of 10 ul per ml
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A 1 kb insert (i) is ligated to 5 kb vector (v)in molar ratio of i:v of 5:iin a reaction volume of 1 ml at final dna concentration of 10 ul per ml
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