Biology, asked by venkatpranesh1660, 10 months ago

(a) A recombinant vector with a gene of interest inserted within the gene of α-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.
(b) Why is this method of selection referred to as "insertional inactivation"?

Answers

Answered by marvelpotter
4

Answer:

a. Chromogenic substrate is used to identify recombinants and non-recombinants. The bacteria are grown in a medium with chromogenic substrate. Recombinants show no blue coloration while non-recombinants show blue coloured colonies.

b. This method is called “insertional inactivation” because the insertion of recombinant-DNA within the coding sequence β-galactosidase enzyme site results into inactivation of the enzyme.

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Answered by Anonymous
9

Answer:

(A) When the foreign gene is inserted within alpha - galactosidase gene, the enzyme alpha - galactosidase gets inactivated. The bacteria are grown on a chromogenic substrate. The non - recombinants will produce blue-colored colonies while the recombinants will produce white / colorless colonies because of the inactivation of the gene alpha - galactosidase.

(b) Insertional inactivation is the loss of the function of a gene such as alpha-galactosidase gene in plasmid due to insertion of foreign DNA / transgene within it. This tool is used to select recombinants.

Explanation:

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