(a) A recombinant vector with a gene of interest inserted within the gene of α-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones.
(b) Why is this method of selection referred to as "insertional inactivation"?
Answers
Answer:
a. Chromogenic substrate is used to identify recombinants and non-recombinants. The bacteria are grown in a medium with chromogenic substrate. Recombinants show no blue coloration while non-recombinants show blue coloured colonies.
b. This method is called “insertional inactivation” because the insertion of recombinant-DNA within the coding sequence β-galactosidase enzyme site results into inactivation of the enzyme.
Hope it helps..
✌
Answer:
(A) When the foreign gene is inserted within alpha - galactosidase gene, the enzyme alpha - galactosidase gets inactivated. The bacteria are grown on a chromogenic substrate. The non - recombinants will produce blue-colored colonies while the recombinants will produce white / colorless colonies because of the inactivation of the gene alpha - galactosidase.
(b) Insertional inactivation is the loss of the function of a gene such as alpha-galactosidase gene in plasmid due to insertion of foreign DNA / transgene within it. This tool is used to select recombinants.
Explanation:
hope it helps you
mark me brainliest