Question

a] Answer in brief:

i) Write the double staining procedure of anatomical sections.

ii) How can you differentiate unilocular and plurilocular sporangium of Ectocarpus sp.​

Answer 1

Explanation:

Ectocarpus sporophytes can produce unilocular sporangia regardless of ploidy or sexuality. In these images, basal filament cells are highlighted in a dotted white box and sporangia lie to the right of this box. Nuclei were stained with Hoechst 33342 and imaged using two-photon microscopy. Mature plurilocular sporangium (a) and unilocular sporangium (b) of a sexually derived sporophyte (SP), showing diploid basal filament cells. Mature plurilocular sporangium (c) and unilocular sporangium (d) of an asexually derived parthenosporophyte (pSP), again showing diploid basal filament cells. Mature plurilocular sporangium (e) and unilocular sporangium (f) of an asexually derived parthenosporophyte, this time showing less brightly stained, haploid basal filament cells; (g) ratio of locular nuclear content to somatic nuclear content in sporophytes and haploid parthenosporophytes, with * indicating a significant (P < 0.05) difference from unity; n = 8–12 filaments for each value.

Answer 2

Answer:

(i) A mixture of two dyes, each of which stains different portions of a tissue or cell.

(ii) The main difference between unilocular and plurilocular sporangia is that unilocular sporangia consist of a single enlarged cell, which undergoes meiosis followed by mitosis, forming haploid zoospores whereas plurilocular sporangia consist of a large number of cuboidal cells, which develops into diploid zoospores.

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