Question

A biotechnologist wanted to create a colony of E.coli possessing the plasmid pBR322, sensitive to Tetracycline. Which one of the following restriction sites would he use to ligate a foreign DNA?
a.)Sal I
b.)Pvu I
c.)EcoRI
d.)Hind III

Answer 1

Answer:

c.)EcoRI

Explanation:

it is the best restriction site

Answer 2

Answer:

Explanatio

ANS: tetracycline has 2restriction sites sal1 and bamh1.

So he should ligate the foreign dna at sal 1