A child wanted to separate the mixture of dyes constituting a sample of ink. He marked a line by the ink on the filter paper and placed the filter paper in a glass containing water as shown in the figure. The filter paper was removed when the water moved near the top of the filter paper.
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Paper Chromatography can be used to separate the pigments present in chlorophyll.
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1 If the ink contains three different coloured components , we see spots of different colours at different heights on the filter paper .The most soluble in water appears in the highest position , the less soluble in water appears in the middle position and the least soluble in water appears at the lowest position on the Chromatograph.
2 Chromatograph
3This technique is used to separate and identify amino acids obtained by hydrolysis of blood.
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