A controlled experiment was conducted to analyze the effects of darkness and boiling on the photosynthetic rate of incubated chloroplast suspensions. The dye reduction technique was used. Each chloroplast suspension was mixed with DPIP, an electron acceptor that changes from blue to clear when it is reduced. Each sample was placed individually in a spectrophotometer and the percent transmittance was recorded. The three samples used were prepared as follows.
Sample 1 – chloroplast suspension + DPIP
Sample 2 – chloroplast suspension surrounded by foil wrap to provide a dark environment + DPIP
Sample 3 – chloroplast suspension that has been boiled + DPIP
Identify and explain the control or controls for the experiment?
Answers
Answer:
Sample 2 – chloroplast suspension surrounded by foil wrap to provide a dark environment + DPIP
Answer:
The dye reduction method was applied. DPIP, an electron acceptor that transforms from blue to clear when it is reduced, was combined with each chloroplast suspension.
Explanation: What you mean by chloroplast suspension?
The pellet should be gently resuspended by being moved up and down in the pipet after being given 2 ml of phosphate buffer to add to it. Once you have a total volume of 8 ml of buffer, combine the diluted suspension with the pipet using a clean Pasteur pipet. You have a suspension of chloroplasts here.
The process of photosynthesis would be stopped by boiling the chloroplast, which would cause it to break and disintegrate. Without light, photosynthesis would not take place if unboiled chloroplast were present in the cuvette. The process of photosynthesis requires light to function.
The thylakoid membranes in chloroplasts are stacked (sometimes called grana). These thylakoid membranes contain pigments involved in photosynthetic reactions, including chlorophyll. The stroma, a fluid rich in protein, has the thylakoid membranes suspended within them.
To learn more about chloroplast suspension refer to:
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