A e.Coli containing light dna molecule was allowed to grow for 100 minutes in n15 medium then what would be the proportion of light and heavy density dna molecule
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Answer:
light DNA= 0% hybrid DNA= 3.3% heavy DNA= 87% (approx.)
Exp
E.coli divides every 20 minutes. After first 20 minutes, when it will replicate in the N15 medium, it is gonna get, 100% hybrid DNA. Proceeding the same way further, after is gonna get 50% hybrid DNA and 50% heavy DNA. Finallyafter 100 mins. it will get 87% heavy DNA and 3.3% light DNA.
Kindly refer NCERT for the concept of replication. :)
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