A gene carried by recombinant DNA is
cloned when
Answers
Answer:
After insertion of the foreign DNA, the plasmid or phage vector is re-introduced into a bacterial cell. The growing bacterial culture replicates the foreign DNA, along with the vector, in hundreds of copies per cell. This process yields multiple, identical clones of the original recombinant molecule.
Explanation:
Recombinant DNA and gene cloning are essential tools for research in molecular microbiology and medicine. They have many medical applications, including development of new vaccines, biologics, diagnostic tests and therapeutic methods.
The genetic material of bacteria and plasmids is DNA. Bacterial viruses (bacteriophages or phages) have DNA or RNA as genetic material. The two essential functions of genetic material are replication and expression. Genetic material must replicate accurately so that progeny inherit all of the specific genetic determinants (the genotype) of the parental organism.
Replication of chromosomal DNA in bacteria starts at a specific chromosomal site called as the origin and proceeds bidirectionally until the process is completed. When bacteria divide by binary fission after completing DNA replication, the replicated chromosomes are partitioned into each of the daughter cells.
The origin region specifically and transiently associate with the cell membrane after DNA replication has been initiated, leading to a model whereby membrane attachment directs separation of daughter chromosomes (the replicon model).
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Recombinant DNA is the general name for a piece of DNA that has been created by combining at least two fragments from two different sources. ... Recombinant DNA molecules are sometimes called chimeric DNA, because they can be made of material from two different species, like the mythical chimera