Question

A genetically engineered form of brinjal,known as Bt-brinjal has been developed wat is its objective?pls xplain

Answer 1

The Bt brinjal is a suite of transgenic brinjals(also known as an eggplant or aubergine) created by inserting a crystal protein gene(Cry1Ac) from the soil bacterium Bacillus thuringiensis into the genome of various brinjal cultivars. The insertion of the gene, along with other genetic elements such aspromoters, terminators and an antibiotic resistance marker gene into the brinjal plant is accomplished using Agrobacterium-mediated genetic transformation. The Bt brinjal has been developed to give resistance againstlepidopteron insects, in particular the Brinjal Fruit and Shoot Borer (Leucinodes orbonalis)(FSB). Mahyco, an Indian seed company based in Jalna, Maharashtra, has developed the Bt brinjal.

The genetically modified brinjal event is termed Event EE 1, and Mahyco have also applied for approval of two brinjal hybrids. The Event EE 1 was introgressed by plant breeding into various local varieties byUniversity of Agricultural Sciences, Dharwadand Tamil Nadu Agricultural University, Coimbatore. Some of the cultivars of brinjal include: Malpur local, Manjari gota, Kudachi local, Udupi local, 112 GO, and Pabkavi local.[1] It was approved for commercialization in India in 2009, but - after an apparent public outcry and rounds of debates in which representatives from Mahyco, the scientific community, and NGO's spoke on the topic - the then Indian Environment Minister, Jairam Ramesh, facilitated a moratorium on its release until further, unspecified, tests were conducted. Bt brinjal was approved for commercial release in Bangladesh in 2013.

Answer 2

Objectives

When fruit and shoot borer larvae feed on Bt brinjal plants, they ingest the Bt protein Cry1Ac along with plant tissue. In the insect gut, which is alkaline with a pH >9.5, the protein is solubilized and activated by gut proteases. The Bt protein binds to specific receptor proteins present in the insect membrane, resulting in pore formation in the membranes. This leads to disruption of digestive processes, paralysis, and subsequent death of the fruit and shoot borer larvae.[3]
What is Bt brinjal ?
Bt brinjal is a suite of transgenic brinjals (also known as an eggplant or aubergine) created by inserting a crystal protein gene (Cry1Ac) from the soil bacterium Bacillus thuringiensis into the genome of various brinjal cultivars. The insertion of the gene, along with other genetic elements such as promoters, terminators and an antibiotic resistance marker gene into the brinjal plant is accomplished using Agrobacterium-mediated genetic transformation. The Bt brinjal has been developed to give resistance against lepidopteron insects, in particular the Brinjal Fruit and Shoot Borer (Leucinodes orbonalis)(FSB). Mahyco, an Indian seed company based in Jalna, Maharashtra, has developed the Bt brinjal.