Question

A human cell contains multiple lipids such as phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, triglycerides and diglycerides. Design TLC experiments to separate these lipids from human cell extract?

Answer 1

Answer:

Thin-layer chromatography (TLC) is a technique that has been routinely used for the separation and identification of lipids. Here we describe an optimized protocol for the steady state labeling, separation, and quantification of yeast phospholipids using 1-D TLC.

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Answer 2

Phosphatidylcholines are generally the most abundant phospholipid class in a membrane. They also constitute the major phospholipid class contained in lipoproteins, biliary lipid aggregates and lung surfactant