Question

A. Identify both the cellular component and the location of the component that is responsible for producing the luciferase protein from mRNAs transcribed in the plasmid-containing T lymphocytes. Explain what dictates to the lymphocytes the correct order in which amino acids should be linked to form the luciferase protein.
B. Identify the independent variable in the experiment described. Identify the plasmid that was used as a negative control for luciferase activity. Justify including the plasmid with the non- CD3γ active promoter in the experiments.
C. Predict the most likely observed level of luciferase activity if plasmid pCDγ3-789 is introduced into nonlymphoid cells such as a line of kidney tissue cells. Provide reasoning to justify your prediction.

Answer 1

Plasmids

The cellular components are necessary for the production of the protein luciferase.

Explanation:

• Sikwarms and fireflies are used to produce that protein.
• Moreover, the correct coding and sequencing of the protein are also necessary for protein production.
• The control of the protein production of protein feasible under feasible conditions depends on the concentration of the protein in the cytoplasm.

Answer 2

Answer:

Explanation:

Luciferases are proteins with enzymatic activity that, in the presence of ATP, oxygen, and the appropriate substrate (typically luciferin), catalyze the oxidation of the substrate in a reaction that results in the emission of a photon.

lymphocytes the correct order in which amino acids should be linked to form the luciferase protein.