Question

A living tissue such as liver is homogenised with trichloracetic acid and filtered. The retentate fraction obtained will contain

Answer 1

Explanation:

The retentate is acid insoluble fraction mostly inorganic compounds

Answer 2

The retentate fraction obtained will contain inorganic compounds.

Separation of cytoplasmic constituents:

• Cytoplasmic contents are ground using mortar and pestle and mixed with trichloroacetic acid, then strained.
• This results in the separation of compounds between acid-soluble and acid-insoluble fractions.
• The acid-soluble pool contains organic compounds like flavenoids and the acid-insoluble pool contains inorganic compounds.
• The retentate part contains polysaccharides, amino acids, nucleic acids, etc.

This is how analysis of living tissue is done via trichloroacetic acid.