Question

A mixture of lysine, glycine, alanine, isoleucine and glutamic acid are separated by ionic exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2: a) with a cation exchange resin? b) with an anion exchange resin?

Answer 1

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Answer 2

Explanation:

☑️ A mixture of lysine, glycine, alanine, isoleucine and glutamic acid are separated by ionic exchange chromatography. What is the order of elution of these amino acids if you use gradient buffer system from pH 10 to pH 2:

a) with a cation exchange resin

b) with an anion exchange resin.