A mutant of E. coli with a heat sensitive DNA ligase (25°C permissive, 37°C non-permissive has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of [^3H]-thymidinein the mutant would demonstrate the accumulation of short segments ofa. unlabeled DNA at 25°C and at 37°Cb. unlabeled DNA at 25°C but not at 37°Cc. radioactive DNA at 37°C but not at 25°Cd. radioactive DNA at 25°C but not at 37°C
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A mutant of E. coli with a heat-sensitive DNA ligase (25oC permissive, 37oC nonpermissive) has been used to show that DNA synthesis is discontinuous. Examination of DNA replication in the presence of [3H]-thymidine in the mutant would demonstrate which of following?
(A) The accumulation of short segments of unlabeled DNA at 25oC and at 37oC.
(B) The accumulation of short segments of unlabeled DNA at 25oC but not at 37oC.
(C) The accumulation of short segments of radioactive DNA at 37oC but not at 25oC.
(D) The accumulation of short segments of radioactive DNA at 25oC but not at 37oC.
(E) The incorporation of short fragments of radioactive DNA into longer ones at 25oC and at 37oC.
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