Question

A. Name the selectable markers in the cloning vector pBR322? Mention the role they play.
B. Why is the coding sequence of an enzyme β-galactosidase a preferred selectable marker in comparison to the ones named above?

Answer 1

Answer:

A.The plasmid pBR322 has two selectable markers: ampR and tetR , ampR is resistance to ampicillin and tetR is resistance to tetracycline. Selectable marker is a gene that select by allowing transformed cells to survive on media contining toxic level of selective agent.