A new near-infrared (nir) fluorescent probe based on 2-methylchromone was exploited for relay recognition of cu2+ 12 and biothiols. The mechanism involved in 13 complex between metal ion and probe, and subsequent displacement reaction, which 14 make the probe can detect gsh by nir fluorescence turn on emission. Probe showed 15 a fluorescence band at 718 nm in pbs-etoh (3:2, v/v, 10 mm pbs, ph 7.4) solution.
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