Question

A novel method for expansion and differentiation of mouse tracheal epithelial cells in culture

Answer 1

Air-liquid interface (ALI) cultures of mouse tracheal epithelial cells (MTEC) are a well-established model to study airway epithelial cells, but current methods require large numbers of animals which is unwanted in view of the 3R principle and introduces variation. Moreover, stringent breeding schemes are frequently needed to generate sufficient numbers of genetically modified animals. Current protocols do not incorporate expansion of MTEC, and therefore we developed a protocol to expand MTEC while maintaining their differentiation capacity. 


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Answer 2

Answer:

We have defined conditions for primary culture of MTEC that result in rapid proliferation and generation of a highly differentiated epithelium