Question

A pcr reaction that continues for 30 cycles will produce approximately how many pcr products from a single template dna molecule? A) 64 b) 128,000 c) approximately 1 million d) approximately 1 billion

Answer 1

ans

c) is right answer I million

Answer 2

A pcr reaction that continues for 30 cycles will produce approximately 1 billion pcr products from a single template DNA molecule.

• 30 cycles will produce approximately 230 PCR products, which is 1.07 billion.
• Polymerase chain reaction (PCR) is a method used to amplify DNA.
• PCR involves using short synthetic DNA fragments called primers to select a segment of the genome to be amplified.
• It then goes for  multiple rounds of DNA synthesis to amplify that segment.
• PCR is fundamental to many of the procedures used in genetic testing and research today.
• PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications.
• All PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase.
• Taq polymerase is an enzyme originally isolated from the thermophilic bacterium Thermus aquaticus.
• Applications of the technique include DNA cloning for sequencing, gene cloning and manipulation, gene mutagenesis etc.

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