A Plasmid containing strain of E.coli is used to produce recombinant protein in a 250 - liter fermenter. The
probability of plasmid loss per generation is 0.005. The Specific Growth rate of Plasmid Free Cells is 1.47 hour. The specific growth rate of plasmid bearing cells is 1.2 hour. Estimate the fraction of plasmid bearing cells after 18 hour growth if the inoculum contains only cells with plasmid. ?
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