Biology, asked by ShravaniMR9137, 1 year ago

A plasmid dna and linear dna both of same size have one site for restriction endonuclease when cut

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Answered by ayushanand8947
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It is because plasmid is a circular DNA molecule. When cut with enzyme, it becomes linear but does not get fragmented. Whereas, a linear DNA molecule gets cut into two fragments. Hence, a single DNA band is observed for plasmid while two DNA bands are observed for linear DNA in agarose gel.

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