Question

A protein X was fused with GFP in a vector and expressed in E. coli. The length of the protein X is 1000 amino
acid residues and the molecular weight of GFP is 27 kilodaltons. What is the total approximate molecular
mass of the fusion protein in daltons?
​

Answer 1

Answer:

26,870 daltons

With GFP, the primary structure is 239 amino acids with a total molecular weight of 26,870 daltons, or 26.9 kD.

Answer 2

Answer:

Explanation:

Fusion protein = Protein'X'+GFD

Molecular weight of fusion protein=M.W of protein 'X'+M.W of GFD

M.W of protein'X'=1000*110(mol wt of aa~110 da)

M.W of GFD=27 da(gn)

=(1000*110)+(27*1000)

=110000+27000

=137000 Da