A ratiometric fluorescent probe based on boron dipyrromethene and rhodamine forster resonance energy transfer platform for hypochlorous acid and its application in living cells
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We have developed a ratiometric fluorescent probe BRT based on boron dipyrromethene (BODIPY) and rhodamine-thiohydrazide Förster resonance energy transfer (FRET) platform for sensing hypochlorous acid (HOCl) with high selectivity and sensitivity. The probe can detect HOCl in 15 s with the detection limit of 38 nM. Upon mixing with HOCl the fluorescence colour of probe BRT changed from green to orange. Moreover, probe BRT was applied to successfully monitor HOCl in living RAW 264.7 cells.
Ratiometric fluorescence probes for HOCl could be also devel- oped using single molecular FRET technique with coumarin [73,221e224], dansyl [225], BODIPY [226] or naphthalene de- rivatives [70] as the energy donor and rhodamine as the energy acceptor. Probe 130 (Fig. 11) was developed by Yuan et al. as the first example of FRET-based ratiometric fluorescence probe for quanti- tative detection of HOCl [221]. ...