A recombinant DNA is formed when sticky ends of vector DNA and foreign. DNA join. Explain how the sticky ends are formed and get joined.
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A recombinant DNA is formed when sticky ends of the vector. Arecombinant DNA is formed when sticky ends of the vector DNA and theforeign DNA join. ... When restriction endonuclease cuts down the strands of the two DNA from different sources, helix opens up leading to theformation sticking ends.
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Explanation:
- Recombinant DNA is the DNA which is formed by the humans through restriction enzymes and vectors which can cut the desired DNA fragments.
- The restriction enzymes act as molecular scissors as they make cut in the DNA sequence in two ways: sticky ends and the blunt ends.
- The sticky ends are produced when the enzyme makes the cut in the palindromic sequence in a way they can be easily ligated later.
- The enzyme-like EcoRI makes the cut in the palindromic sequence GAATTC by making a cut between G and A in both strands.
- These ends can easily pair up by complementary base pairing rule.
To learn more:
1. Recombinant technology: https://brainly.in/question/12341460
2. Restriction enzymes: https://brainly.in/question/15397355
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