Question

A recombinant DNA molecule can be produced in the absence of the
following:
a. Restriction endonuclease
b. DNA ligase
c. DNA fragments
d. E.coli


NCERT Class XII
Biology - Main Course Book

Chapter 11. Biotechnology: Princeples and Processes

Answer 1

The answer is: (d) E.coli.

Answer 2

Answer:

A recombinant DNA molecule……

Explanation:

Lets first understand what actually do we mean by a recombinant DNA molecule. The word Recombinant DNA refers to a strand of DNA that has been made by combining minimum of two different strands. This feature of recombinant is in fact possible  only when the DNA strand or molecules taken from all other organisms are similar to each other. Under such circumstance you need to keep in mind that they need to be identical in all features in order to combine. They can only differ in Nucleotide sequence.

So now when looking at the options that you have provided then we can say that

1. Restriction Endonuclease is a DNA factor that cannot be the correct answer.

2. DNA ligase is also a DNA factor so it will never be the correct option.

3. DNA fragments certainly is not possible, so we rule out this option as well.

4. E.Coli….. it is not a DNA factor, it is a name given to a microbe. So this certainly has to be the correct answer.

So the right answer for your question is E.Coli.