A sample of 2,4-hexadiene in methanol in a 2.5 cm cell shows an absorbance of 0.65 at 240.0 nm. What is the concentration of the diene? The molar absorption at this wavelength is 12100 dm3 mol1 cm1.
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Answer..
Absorbance of the diene solution, A = 0.65
Absorbance of the diene solution, A = 0.65Path length of the cell, l = 2.5 cm
Absorbance of the diene solution, A = 0.65Path length of the cell, l = 2.5 cmMolar absorptivity of the cell, ε = 12100 dm³ mol⁻¹ cm⁻¹
Absorbance of the diene solution, A = 0.65Path length of the cell, l = 2.5 cmMolar absorptivity of the cell, ε = 12100 dm³ mol⁻¹ cm⁻¹Let concentration of the diene be 'c'.
Absorbance of the diene solution, A = 0.65Path length of the cell, l = 2.5 cmMolar absorptivity of the cell, ε = 12100 dm³ mol⁻¹ cm⁻¹Let concentration of the diene be 'c'.As per Beer-Lambert law,
Absorbance of the diene solution, A = 0.65Path length of the cell, l = 2.5 cmMolar absorptivity of the cell, ε = 12100 dm³ mol⁻¹ cm⁻¹Let concentration of the diene be 'c'.As per Beer-Lambert law,A = c × ε × l
Absorbance of the diene solution, A = 0.65Path length of the cell, l = 2.5 cmMolar absorptivity of the cell, ε = 12100 dm³ mol⁻¹ cm⁻¹Let concentration of the diene be 'c'.As per Beer-Lambert law,A = c × ε × l∴ Concentration of the diene, c
= 2.14 × 10⁻⁵ dm⁻³ mol
= 2.14 × 10⁻⁵ dm⁻³ mol = 2.14 × 10⁻⁵ mol L⁻¹ [∵ 1 L = 1 dm³]
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