Question

A scientist wants to express human protein Y in bacteria. For effective expression of this protein he should use A. promoter of human gene Y.
B. promoter of bacterial gene. C. operator of any human gene. D. operator of any bacterial gene.

Answer 1

Answer:

The correct answer is (B) promoter of the bacterial gene.

For effective expression of this protein, he should use a promoter of the bacterial gene.

Bacteria have the ability to produce foreign proteins using their own gene expression machinery. However, to use this machinery, a strong promoter is required. This facilitates a greater production of the protein of interest within the bacteria.

Answer 2

For effective expression of “human protein Y in bacteria” scientist need to use “promoter of human gene Y”.

Answer: Option A

Explanation:

The main difference between promoter and operator is that, promoter is the sequence of gene which is read and bind by the RNA polymerase to start transcription. The operator is collection of genes which are “under the control of one promoter”. As the scientists wants to express the human protein Y, he must first transcript the protein such that it can be effectively expressed. This he should use “promoter of human gene Y”.