A selectable marker is used in the selection of recombinants on the basis of their ability to produce colour in the presence of the chromogenic substrate. a) Mention the name of the mechanism involved. b)Which enzyme is involved in the production of the colour?c)How is it advantageous over using antibiotic resistant gene as a selectable marker?
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a) The name of mechanism is insertional inactivation.
b) The name of enzyme involved colour production is β- galactosidase
c) Two plates with different antibiotics are required for simultaneous plating for selection of recombinant due to antibiotic inactivation but here chromogenicsubstrate are used which gives blue coloured colonies when there is no insert
b) The name of enzyme involved colour production is β- galactosidase
c) Two plates with different antibiotics are required for simultaneous plating for selection of recombinant due to antibiotic inactivation but here chromogenicsubstrate are used which gives blue coloured colonies when there is no insert
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