Question

A solution containing the mixture of AMP and GMP gave an absorbancy index of 0.652 at 260 nm and 0.248 at 280 nm given molar absorbancy index below.Calculate the concentration of AMP and GMP in solution if AMP A260 nm = 15.4×10^3 per M^-1 cm^-1 at A280 = 2.5×10^3 M^-1 cm ^-1 and GMP:A260 = 11.7×10^3 M^-1 cm^-1 and A280 = 7.7 ×10^3 M^-1 cm^-1

solve complete ..........​

Answer 1

Answer:

Protein analysis is needed to determine if a sample solution contains the desired protein. For example, measuring the absorbance of a protein sample at 280 nm with a spectrophotometer is a rapid and straightforward method. In many bio-analytical applications, it is important to estimate or accurately determine the concentration of sample solutions containing purified biomolecules such as oligonucleotides, peptides or proteins. Often this information is needed or used for the design of down-stream experiments in analytical chemistry, biology, biochemistry, biophysics, medicinal chemistry, and pharmacy.

Absolute quantification of protein samples to determine accurate protein mass or concentration is often required to estimate recovery at different purification stages, to measure the specific activity of a protein, and to prepare known amounts of protein samples for analytical analysis. In general, calibrated sets of external standards containing known amounts of a specific protein such as bovine serum albumin (BSA) are often used to create calibration curves