A solution of tryptophan has an absorbance at 280 nm of 0.54 in a 0.5 cm pathlength cuvette. Given the absorbance coefficient (e) for tryptophan is 5.4x10°L mol2 cm-t, the concentration of the solution is
a. 0.2 mm
b. 20 mm
c. 1 x 10-3 M
d. 0.1 mm
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How do you find the concentration of a solution with absorbance?
In order to derive the concentration of a sample from its absorbance, additional information is required.
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Absorbance Measurements – the Quick Way to Determine Sample Concentration
Transmission or transmittance (T) = I/I0 ...
Absorbance (A) = log (I0/I) ...
Absorbance (A) = C x L x Ɛ => Concentration (C) = A/(L x Ɛ)
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