A strain of mold was grown in a batch culture on glucose and the following data were obtained.
Time (h)
0
9
16
23
30
34
36
40
Cell concentration (g/l)
1.25
2.45
5.1
10.5
22
33
37.5
41
Glucose concentration
(g/l)
100
97
90.4
76.9
48.1
20.6
9.38
0.63
i) Calculate the maximum net specific growth rate (unet).
ii) Calculate the apparent growth yield (Yx/s).
iii) What maximum cell concentration could one expect if 150 g of glucose was used with the same size inoculum?
Answers
Answer:
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specific growth rate, µ = (1/X)*(dX/dt)
Explanation:
- Because it depicts the dynamic activity of microorganisms, the specific growth rate is the most critical parameter to regulate during fermentation.
- The use of a specific growth rate as a control parameter implies that appropriate amounts of the substrate are present, and so it may be used as an indirect tool for controlling the extracellular environment.
- By formula, we have where X is the cell mass concentration and t is the time spent in the bioreactor system.
- The yield is affected by the amount of substrate used (an important consideration in commercial cultures where you have to ask how much product you are getting from the expensive substrate you are adding).
- This is the yield coefficient (Y), which is the ratio of biomass produced (in grammes) to substrate consumed
- Due to the physical differences between unicellular bacteria or yeasts and apically-elongating, non-fragmenting, hyphal fungi, culture absorbance is a less reliable approach for estimating the biomass of filamentous fungus than it is for bacteria or yeasts.
- Nonetheless, for most fungal growth assays, it is a non-destructive and useful method.