Question

A student performed chromatography to separate the components of a water in-soluble marker He used water as a solvent and noticed the spot did not rise up why do you think that happened what solution would you give to solve this problem​

Answer 1

Explanation:

Thin layer chromatography (TLC) is a chromatographic technique used to separate the components of a mixture using a thin stationary phase supported by an inert backing. It may be performed on the analytical scale as a means of monitoring the progress of a reaction, or on the preparative scale to purify small amounts of a compound. TLC is an analytical tool widely used because of its simplicity, relative low cost, high sensitivity, and speed of separation.TLC functions on the same principle as all chromatography: a compound will have different affinities for the mobile and stationary phases, and this affects the speed at which it migrates. The goal of TLC is to obtain well defined, well separated spots.

Answer 2

Explanation:

Paper chromatography is a technique used to separate the compounds in which cellulose paper acts as a stationary phase and a solvent is used as a mobile phase.

• Cellulose is polar in nature and water also is a polar solvent therefore there is no distinction by which we can separate the molecules.
• Hence water is not used in chromatography water causes the paper to swell up and dissolve.
• Therefore spot did not rise to avoid this, non-polar solvents being used.