Question

A student wanted to prepare temporary mount of a leaf peel to study the distribution of stomata.Name the chemical used for mounting.What is the signification of using this substance instead of water for mountingi

Answer 1

Saffranin and glycerine is used for this purpose.
I hope this helps!!

Answer 2

The chemical that is used for mounting of a leaf peel is - Glycerine.

Safranin is a chemical which is used for staining of the leaf peel and Glycerine is used for mounting the stained leaf peel in order to observe the distribution of the stomata.

Safranin is an azonium compound of 2,8-dimethyl-3,7-diamino-phenazine. It stains the cells to give a red colour to the nuclei.

Glycerin, also known as glycerol is used as a mounting agent. It is used instead of water because glycerine is a good medium that keeps the specimen, the slide, and the coverslip held together. It is a good dehydrating agent and prevents the formation of bubbles. Also, it reflects light, which gives a clear and accurate picture of the specimen under the microscope. It is also a good agent used for protecting the specimens.