A student wanted to separate the mixture of dye constituting a sample of ink.
He marked a line on the filter paper and placed the filter paper in a glass
containing water as shown in figure. The filter paper was removed when the
water moved near the top of the filter paper.
(i)What would you expect to see, if the ink contains three different coloured
components?
(ii)Name the technique used by the student.
(iii)Suggest one more application of this technique.
iv) How Tyndall effect can be observed in the canopy of a dense forest.
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a) If the ink contains three different coloured components , we see spots of different colours at different heights on the filter paper as seen in the figure . The most soluble in water appears in the highest position , the less soluble in water appears in the middle position and the least soluble in water appears at the lowest position on the chromatograph.
(b) Chromatography
(c) This technique is also used to separate and identify amino acids obtained by hydrolysis of blood.
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