Question

a student wants to insert a novel gene in a plant that can provide insecticide resistance.please elaborate different steps involved in the instruction of recombinant vector and its selection with the help of well label diagram ?​

Answer 1

Answer:

sorry l don't about this

Answer 2

Using DNA technology, researchers were able to track down the gene that creates insect-killing Bt proteins and transfer them to crop plants.

Bt technology is depicted in Figure 

First, scientists must find a Bt strain that kills the desired insect. The gene that creates the deadly protein is then isolated. The gene that confers resistance to a chemical (typically an antibiotic or herbicide) is removed from the Bt bacteria, and a gene imparting resistance to a chemical (usually an antibiotic or herbicide) is attached, which will be useful in a later phase.

In plant cells, the Bt gene with the resistance gene linked is introduced. Scientists must now figure out which plant cells have successfully gotten the Bt gene and have been transformed. Any plant cell with the Bt gene also has to have the resistance gene connected to it. The plant cells are grown in the presence of antibiotics.or pesticide, and then pick out the plant cells that aren't damaged. Tissue culture is used to grow these genetically modified plant cells into full-fledged plants. Because the transformed plants now have the same gene as Bt bacteria, they produce the same lethal Bt protein.

Transferring insect resistance genes from Bt to crop plants is currently being researched. Corn, cotton, and potatoes are just a few of the commercial crops that have been bred to resist Bt insecticides.