Question

A substantial number of
Azotobacter inoculant gets adsorbed on -​

Answer 1

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Answer 2

Azotobacter

A substantial number of  Azotobacter inoculant gets adsorbed on seeds.

Azotobacter is a genus of usually motile, oval, or spherical bacteria that form thick-walled cysts and may produce large quantities of capsular slime. They are aerobic, free-living soil microbes that play an important role in the nitrogen cycle in nature, binding atmospheric nitrogen, which is inaccessible to plants, and releasing it in the form of ammonium ions into the soil (nitrogen fixation). In addition to being a model organism for studying diazotrophs, it is used by humans for the production of biofertilizers, food additives, and some biopolymers. The first representative of the genus, Azotobacter chroococcum, was discovered and described in 1901 by Dutch microbiologist and botanist Martinus Beijerinck. Azotobacter species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants.

• Bacterization helps to improve plant growth and to increase soil nitrogen through nitrogen fixation by utilizing carbon for its metabolism. Seed Inoculated with Azotobacter helps in the uptake of N, P along with micronutrients like Fe and Zn, in wheat, these strains can potentially be used to improve wheat nutrition.
• Azotobacter species are Gram-negative bacteria found in neutral and alkaline soils, in water, and in association with some plants.
• Flasks are then inoculated with mother culture with the help of inoculating needle aseptically. The flasks are transferred to a shaker and shaking is done for 72-90 hours so as to get optimum growth of bacteria in broth. Bacteria are multiplied by binary method i.e. cell division. This is how Azotobacter is produced in labs.
• Azotobacter Agar (Mannitol) is used for the isolation and cultivation of mannitol-positive Azotobacter species from the soil. It is also useful for the maintenance of Azotobacter species by adding an extra 1% Mannitol to the medium as specified by the American Type Culture Collection.
• Ten cases of Azotobacter keratitis were identified at the Baylor College of Medicine and Cullen Eye Institute, Houston, from 1972 to 1980. Azotobacter is a large, pleomorphic, aerobic, Gram-negative rod of the family Azotobacteraceae.
• It contains a circular chromosome of 4,591,803 bp and six circular plasmids pAcX50a, b, c, d, e, f of 10,435 bp, 13,852bp, 62,783, 69,713, 132,724, and 311,724 bp respectively. The genome has an overall G+C% content of 65.69%.
• General-purpose nutrient media, Difco Tryptic Soy Agar (TSA), gives rise to somewhat smaller, more discrete colonies than does Burk's agar, and the plates are much easier to count. This medium can be used satisfactorily to grow many strains of Azotobacter including A. vinelandii.