acetic nitric reagent for cellulose extraction
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The method involves removing hemicellulose and lignin withacetic/nitric reagent [6]. Crystallinecellulose is resistant to acetic/nitric reagent but becomes disordered upon treatment with 67 % sulphuric acid making monomeric sugars available to be measured by a colorimetric method using anthrone as a dye
The method involves removing hemicellulose and lignin withacetic/nitric reagent [6]. Crystallinecellulose is resistant to acetic/nitric reagent but becomes disordered upon treatment with 67 % sulphuric acid making monomeric sugars available to be measured by a colorimetric method using anthrone as a dye
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Reagents The acetic/nitric reagent is prepared by mixing 150 ml 80% acetic acid and 15 ml concentrated nitric acid. The anthrone reagent is 0.2 gm anthrone (Eastman, catalog No …
Cited by 1737 Related articles
Sitosterol-β-glucoside as primer forcellulose synthesis in plants
L Peng, Y Kawagoe, P Hogan, D Delmer - Science, 2002 - science.sciencemag.org
… treatment with acetic-nitric reagent (28); NCC, noncrystalline cellulose defined as 4-linked glucan not resistant to acetic-nitric reagent … DCB affects synthesis of SG, SCDs, and cellulose … Amounts of the extracthaving equal counts per minute of radiolabel were loaded per lane …
Cited by 390 Related articles
[PDF] usda.gov
Determination of cellulose and apparent hemicellulose in plant tissue by gas-liquid chromatography
JH Sloneker - Analytical biochemistry, 1971 - Elsevier
… The fibrous precipitate was washed twice with the acetic-nitric acid reagent (3 ml) and twice with acetone 2 ml) … The quantity of starch was then equal to the total glucose recovered (calculated as C,Hj005) minus that for cellulose …
Cited by 68 Related articles
[PDF] academia.edu
A rapid and simple method to isolate pure alpha-cellulose
O Brendel, PM Iannetta, D Stewart - Phytochemical Analysis, 2000 - academia.edu
… to the inner wall of the test tube was washed downwards to aid complete extraction: this wasachieved by adding liquid extraction reagent in two … Figure 1. The protocol for purification of cellulose from micro- samples of plant cell wall material using aceticacid:nitric acid for …
Cited by 219 Related articles
Application of a modified BCR sequentialextraction (three-step) procedure for the determination of extractable trace metal contents in a sewage sludge amended soil …
G Rauret, JF López-Sánchez, A Sahuquillo… - Journal of …, 2000 - pubs.rsc.org
… Take 250 mL of this solution (acetic acid, 0.43 mol L −1 ) and dilute to 1 L with distilled water to obtain an acetic acid solution of … (ii) Reagent blank … Clean all the glassware by carefully immersing in warm 0.5 mol L −1 nitric acid for a minimum of 6 h and then rinse with deionised …
Cited by 367 Related articles
Isolation and characterization of cellulosefrom sugarcane bagasse
JX Sun, XF Sun, H Zhao, RC Sun - Polymer degradation and stability, 2004 - Elsevier
… Subsequently, 100 ml 80% aqueous acetic acid (w/w) and 10 ml 70% nitric acid (w/w) was … the oil bath and cooled, 60 ml of distilled water was added, and the reagent was decanted … was then thoroughly washed with distilled water and 95% ethanol to remove the nitricacid and …
Cited by 461 Related articles
[PDF] researchgate.net
Extraction of cellulose and preparation of nanocellulose from sisal fibers
JI Morán, VA Alvarez, VP Cyras, A Vázquez - Cellulose, 2008 - Springer
… Argentina); sodium hydroxide (Anedra, Argentina); hydrogen peroxide (Cicarelli, Argentina), sodium borate (Anedra, Argentina); nitric acid (Cicarelli … All used reagents were analytical grade … 1,745 cm −1 . Taking into consideration that Procedure I required the use of acetic acid as …
Cited by 706 Related articles
Hemicellulosic contamination of acid detergent residues and their replacement by cellulose residues in cell wall analysis
IM Morrison - Journal of the Science of Food and Agriculture, 1980 - Wiley Online Library
… use of ADR values be replaced by CR values. For simplicity the original gravimetric procedure to estimate cellulose using ;the acetic acid-nitric acid reagent was used. A modified version of this technique has been reported14 …
Cited by 57 Related articles
The relation of cellulose and lignin content to the nutritive value of animal feeds
EW Crampton, LA Maynard - the Journal of Nutrition, 1938 - academic.oup.com
… was freed of non- cellulose, organic constituents by digestion with an alcohol- nitric acid reagent. The treatment involved boiling the sample with the reagentfor two and sometimes three suc … In the latter procedure, acetic acid is substituted for the alcohol in the digestion reagent …
SML Rosa, N Rehman, MIG de Miranda… - Carbohydrate …, 2012 - Elsevier
… All solvents and reagents were of analytical grade … liquor to pulp ratio was 25:1 (mL/g). To purify the cellulose pulp, 5.0 mL of 80% (v/v) acetic acid and … mL), distilled water (20 mL), and again 95% ethanol (20 mL) to remove extraction breakdown products and traces of nitric acid …
Cited by 1737 Related articles
Sitosterol-β-glucoside as primer forcellulose synthesis in plants
L Peng, Y Kawagoe, P Hogan, D Delmer - Science, 2002 - science.sciencemag.org
… treatment with acetic-nitric reagent (28); NCC, noncrystalline cellulose defined as 4-linked glucan not resistant to acetic-nitric reagent … DCB affects synthesis of SG, SCDs, and cellulose … Amounts of the extracthaving equal counts per minute of radiolabel were loaded per lane …
Cited by 390 Related articles
[PDF] usda.gov
Determination of cellulose and apparent hemicellulose in plant tissue by gas-liquid chromatography
JH Sloneker - Analytical biochemistry, 1971 - Elsevier
… The fibrous precipitate was washed twice with the acetic-nitric acid reagent (3 ml) and twice with acetone 2 ml) … The quantity of starch was then equal to the total glucose recovered (calculated as C,Hj005) minus that for cellulose …
Cited by 68 Related articles
[PDF] academia.edu
A rapid and simple method to isolate pure alpha-cellulose
O Brendel, PM Iannetta, D Stewart - Phytochemical Analysis, 2000 - academia.edu
… to the inner wall of the test tube was washed downwards to aid complete extraction: this wasachieved by adding liquid extraction reagent in two … Figure 1. The protocol for purification of cellulose from micro- samples of plant cell wall material using aceticacid:nitric acid for …
Cited by 219 Related articles
Application of a modified BCR sequentialextraction (three-step) procedure for the determination of extractable trace metal contents in a sewage sludge amended soil …
G Rauret, JF López-Sánchez, A Sahuquillo… - Journal of …, 2000 - pubs.rsc.org
… Take 250 mL of this solution (acetic acid, 0.43 mol L −1 ) and dilute to 1 L with distilled water to obtain an acetic acid solution of … (ii) Reagent blank … Clean all the glassware by carefully immersing in warm 0.5 mol L −1 nitric acid for a minimum of 6 h and then rinse with deionised …
Cited by 367 Related articles
Isolation and characterization of cellulosefrom sugarcane bagasse
JX Sun, XF Sun, H Zhao, RC Sun - Polymer degradation and stability, 2004 - Elsevier
… Subsequently, 100 ml 80% aqueous acetic acid (w/w) and 10 ml 70% nitric acid (w/w) was … the oil bath and cooled, 60 ml of distilled water was added, and the reagent was decanted … was then thoroughly washed with distilled water and 95% ethanol to remove the nitricacid and …
Cited by 461 Related articles
[PDF] researchgate.net
Extraction of cellulose and preparation of nanocellulose from sisal fibers
JI Morán, VA Alvarez, VP Cyras, A Vázquez - Cellulose, 2008 - Springer
… Argentina); sodium hydroxide (Anedra, Argentina); hydrogen peroxide (Cicarelli, Argentina), sodium borate (Anedra, Argentina); nitric acid (Cicarelli … All used reagents were analytical grade … 1,745 cm −1 . Taking into consideration that Procedure I required the use of acetic acid as …
Cited by 706 Related articles
Hemicellulosic contamination of acid detergent residues and their replacement by cellulose residues in cell wall analysis
IM Morrison - Journal of the Science of Food and Agriculture, 1980 - Wiley Online Library
… use of ADR values be replaced by CR values. For simplicity the original gravimetric procedure to estimate cellulose using ;the acetic acid-nitric acid reagent was used. A modified version of this technique has been reported14 …
Cited by 57 Related articles
The relation of cellulose and lignin content to the nutritive value of animal feeds
EW Crampton, LA Maynard - the Journal of Nutrition, 1938 - academic.oup.com
… was freed of non- cellulose, organic constituents by digestion with an alcohol- nitric acid reagent. The treatment involved boiling the sample with the reagentfor two and sometimes three suc … In the latter procedure, acetic acid is substituted for the alcohol in the digestion reagent …
SML Rosa, N Rehman, MIG de Miranda… - Carbohydrate …, 2012 - Elsevier
… All solvents and reagents were of analytical grade … liquor to pulp ratio was 25:1 (mL/g). To purify the cellulose pulp, 5.0 mL of 80% (v/v) acetic acid and … mL), distilled water (20 mL), and again 95% ethanol (20 mL) to remove extraction breakdown products and traces of nitric acid …
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