activity to show observe the onion peel skin with a neet diagram
with aim,materials,procedure
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Answers
Explanation:
HEY MATE ..
Experiment:Objective:To prepare a stained temporary mount of an onion peel and to record observations and draw labeled diagrams.Apparatus and materials required:An onion, glass slide, watch glass, coverslip, forceps, needles, brush, blade, filter paper, safranin, glycerine, dropper, water, and a compound microscope.
All living organisms are made up of cells. The shape, size and the number of these units vary in organisms. The three major components of a cell are the cell membrane, cytoplasm and nucleus. In a plant cell, a cell wall surrounds the cell membrane.Procedure:1. Take an onion and remove its outermost peel.2. Now cut a small part from an inner scale leaf with the help of a blade.3. Separate a thin, transparent peel from the convex surface of the scale leaf with the help of forceps.
4. Keep this peel in a watch glass containing water?5. Add two drops of safranin stain in the watch glass to stain the peel.6. Take a clean slide and put a drop of glycerine in the centre of the slide.7. With the help of a brush and needle transfer the peel on the slide. Glycerine prevents the peel from drying up.8. Carefully cover it with a coverslip and avoid any air bubble from entering interring the coverslip.9. Remove any excessive glycerine with a filter paper.10. Observe the prepared mount of the peel under the low and high magnification of a compound microscope.
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Observations:A large number of rectangular cells are visible. These cells lie close to each other with intercellular spaces between them. These cells are surrounded by distinct cell walls. These cells have a dark stained nucleus and a large vacuole in the centre.
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Precautions:1. Overstaining and understaining should be avoided.
