Question

Activy to check protein in Raw food

Answer 1

Protein Test Materials

calcium oxide (sold as quicklime in building supply stores)red litmus paper (or another method to test pH) watercandle, burner, or another heat sourcedroppertest tubemilk or other foods to test

Procedure

First, test for milk, which contains casein and other proteins. Once you understand what to expect from the test, you can examine other foods.

Add a small amount of calcium oxide and 5 drops of milk to a test tube.Add three drops of water.Dampen the litmus paper with water. Water has a neutral pH, so it should not change the color of the paper. If the paper does change color, start again usingdistilled water rather than tap water.Carefully heat the test tube in a flame. Hold the damp litmus paper over the mouth of the test tube and observe any color change.If protein is present in a food (positive test for protein), the litmus paper will change color from red to blue. Also, if you smell the test tube, you should be able to detect the odor of ammonia if protein was present. If protein isnot present in food or is in insufficient concentration to produce enough ammonia for the test (negative test for protein), the litmus paper will not turn blue.

Answer 2

Biuret solution is used to identify the presence of protein. Biuret reagent is a blue solution that, when it reacts with protein, will change color to pink-purple.