Question

Adsorption and partition chromatography essay

Answer 1

is a highly efficient analytical technique which primarily relies on separation of components over a stationary phase.

This happens under the influence of momentum force of mobile phase on the components.

But this separation on the stationary phase occurs by two principles viz. adsorption and partition.

Hence the term adsorption chromatography and partition chromatography are specifically mentioned under the principle of separation.

These two types of chromatography are invariably used based on the nature of component and samples to be analyzed.



But it seems that higher or well-developed chromatographic techniques used partition principle for component or molecule separation.

Difference between Adsorption and Partition Chromatography

The difference is based on physical and chemical substance analysed and other mobile and stationary phases used. First we will see

Answer 2

HEY!!!

Difference between Adsorption and Partition Chromatography

Chromatography is a highly efficient analytical technique which primarily relies on separation of components over a stationary phase.

This happens under the influence of momentum force of mobile phase on the components.

But this separation on the stationary phase occurs by two principles viz. adsorption and partition.

Hence the term adsorption chromatography and partition chromatography are specifically mentioned under the principle of separation.

These two types of chromatography are invariably used based on the nature of component and samples to be analyzed.

But it seems that higher or well-developed chromatographic techniques used partition principle for component or molecule separation.

Difference between Adsorption and Partition Chromatography

The difference is based on physical and chemical substance analysed and other mobile and stationary phases used. First we will see

Adsorption chromatography principle:

It is a process of separation of components in a mixture introduced into chromatography system based on the relative differences in adsorption of components to the stationary phase present in the chromatography column.

Here the molecules or components of the mixture travel with different rates due to differences in their affinity towards stationary phase. Adsorption means a physical attachment between the compound and the particles of stationary phase. Based on the nature, polar compounds adsorb with stronger or greater intensity to the polar stationary phase while non-polar compounds adsorb better  to the non-polar stationary phase than polar components.

Hence during separation of components, when we use a polar stationary phase, polar components elute out late due to greater adsorption and non-polar components get out of the column or elute out first. This is exactly reverse on using a non-polar stationary phase.

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