Question

Advantage and disadvantage of phase contrast microscope

Answer 1

ADVANTAGES

Can  be used to view specimen that is unstained .

Can be used to study marine organisms due to its dark space.

Can be used to study live bacterium .

DISADVANTAGES

Dark images are often distorted and inaccurate .

Due to  the thickness of the specimen the images might have artifacts throughout  the image .

Accurate measurements of the specimen cannot be acquired.

Answer 2

Advantages of phase contrast microscope:

i) Unstained specimens can be viewed and imaged.

ii) Live cells or organisms can be imaged in their natural form without fixing or killing them.

iii) Produces images that are information-rich and detailed.

Disadvantages of phase contrast microscope:

i) Not useful to see the natural colours of specimens or if staining is involved.

ii) Each objective has its separate phase contrast filter.

iii) Accurate measurements of samples cannot be acquired to technical. limitations where light cannot penetrate beyond a particular sample thickness.