Advantage of lowry over bradford for protein estimation
Answers
Explanation:
rotein assays
All of our allergen source materials will be delivered with a certificate of analysis which states the protein content (mg/g). For a complete picture we show the protein content of two protein determination assays on our certificates; the BCA (bicinchoninic acid) protein assay and the Bradford protein assay:
What are the disadvantages and advantages of these two protein assays? On this page more information about the BCA and Bradford protein assay methods.
Which method is better?
There isn’t a protein assay method that is either perfectly specific to proteins, or uniformly sensitive to all protein types. Therefore, successful use of protein assays involves selecting the method that is most compatible with the samples to be analysed, choosing an appropriate assay standard, and understanding and controlling the particular assumptions and limitations that remain. Historically, the Lowry (BCA) method is more sensitive than the Bradford method, because the first method is based on protein-copper chelation and secondary detection of the reduced copper. Whereas the Bradford method is based on protein-dye binding and colour shift from 465 to 595 nm. This method actually measures the presence of the basic amino acid residues, arginine, lysine and histidine, which contributes to formation of the protein-dye complex. The arginine, lysine and histidine content in Der p 1 is low (about 10%).