Advantages and disadvantages of direct microscopic count
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high cell concentration required to be countable Advantages of Direct Microscopic Count:(/) no incubation time required Electronic Cell Counter/Coulter Counter:(/) automatically count # of cells in measured volume of liquid (/) advantageous b/c no incubation time Turbidity:cloudiness of a microbe suspension (/) estimation of this = way to monitor bacterial growth Colorimeter/Spectrophotometer:used to estimate turbidity (/) beam of light transmitted through suspension to light-sensitive detector (/) as bacteria # increases, less light reaches detector Percentage of Transmission:change of light that registers on colorimeter’s scale Metabolic Activity:indirect way to estimate bacterial # (/) assumes amount of certain metabolic product = in direct proportion to # of bacteria present Dry Weight:used for filamentous bacteria & molds (/) involves fungus filtered to remove extraneous material, dried in desiccator & then weighedAlexandria Toledo

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