Question

advantages and disadvantages of tall and drawf variety

Answer 1

Explanation:

The possible reasons behind choosing primer length exceeding 30 base pairs could be:

1) Mispairing of primer to site other than target site.

2) Forward primer might anneal to the strand of reverse primer, if more than 4-5 base are exactly same in the sequence forming primer dimer.

(It is therefore advised to avoid repetitive long repeats of bases while designing primer)

3) Primer might also bend and pair with the bases in the same strand forming hairpin loop structure.

Any of the above reasons could decrease the efficiency of your PCR !

Answer 2

Answer:

Generally, there are more disadvantages to the tall organizational structure than there are advantages. In a tall organization, there will be a large number of managers, and each manager will usually be responsible for a small group of employees. This means that the span of control of each manager is very narrow.