Question

Advantages of 2d page over 1d page

Answer 1

Answer:

2D PAGE is the classical approach to separate and visualize many proteins from complex proteomics samples. However, 2D PAGE has some known disadvantages. As an example, hydrophobic membrane proteins precipitate during IEF and are almost never observed. So most likely, you will only observe proteins within the pI range of the gel, typically pH 4-7, or 3-10, and the MW range of proteins in the gel is approximately 10-130 kDa [1-3]