After acid treatement decalcified tissue has neutralised by
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Decalcification is carried out after the specimen has been thoroughly fixed and prior to .... Strong acids such as hydrochloric or nitric acid at concentrations up to 10% .
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✔✔ EDTA is a chelating agent, and it can be made 10% solution with distilled water, pH 7.4. ... The fresh solution is changed several times or once a week. After decalcification, samples can be routinely processed and embedded in paraffin.✔✔
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