Question

After dimerization, TCF transcription factors bind DNA sequences known as E-box motifs. These have the consensus sequence CANNTG (where N is any base), with the central two nucleotides providing specificity of binding. You amplify radiolabeled segments of DNA containing the E-box motif for TCF4 binding. You then perform an Electrophoretic Mobility Shift Assay by mixing the radiolabeled DNA segment with either wild type TCF4 protein or TCF protein isolated from Patient #3 above. a) What result do you expect from your EMSA (2 marks) and why? (1mark) b) How would you expect your EMSA gel to look if you ran it under denaturing conditions? (1 mark) Why​

Answer 1

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