Science, asked by js1206Jaskirat, 1 year ago

After staining with methylene blue why mitochondria are not visible under microscope??

Answers

Answered by sharanjeet185

Mitochondria are not visible under a compound microscope when they are stained with methylene blue .But,in order to observe mitochondria we stain them with Janus Green B Stain that is a vital stain,under the electron microscope.They are really small to be seen under compound microscope

js1206Jaskirat: Please tell the reason

sharanjeet185: According to me, it should be:

sharanjeet185: That mitochondria are living organelles and thus we need a living stain to observe them.also, they are small and can't be seen under a compound microscope may be

sharanjeet185: The Janus green stain changes colour in presence of oxygen and as mitochondria are involved in production of ATP they must be changing the colour:)

js1206Jaskirat: thanks