Question

Agrobacterium based gene transfer

Answer 1

Answer:

Agrobacterium transfers a particular gene segment called transfer DNA (T-DNA) from the Ti plasmid. After T-DNA is stably integrated into the chromosomal DNA in the nucleus of the host plant, genes for opine synthesis and tumor-inducing factors on the T-DNA are transcribed in the infected cells.

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Answer 2

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• Agrobacterium is a type of vector by which specific genes of nematode are introduced into the host plant.

• The introduction of DNA was such that it produced both sense and anti-sense RNA is the host cells. These two RNAs being complementary to each other for- me a dsRNA that intiated RNAi and, therefore, silenced the specific mRNA of the nematode.

• The result was that the parasite could not survive in a transgenic host expressing specific interfering RNA. The transgenic plant thus got it self protect from the parasite.