Agrobacterium, RNA/, crylAc, Meloidegyne.
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RNA interference (RNAi) is a powerful reverse genetic tool to study gene function. The data presented here show that Agrobacterium rhizogenes-mediated RNAi is a fast and effective tool to study genes involved in root biology. The Arabidopsis gene KOJAK, involved in root hair development, was efficiently knocked down. A. rhizogenes-mediated root transformation is a fast method to generate adventitious, genetically transformed roots. In order to select for co-transformed roots a binary vector was developed that enables selection based on DsRED1 expression, with the additional benefit that chimaeric roots can be discriminated. The identification of chimaeric roots provided the opportunity to examine the extent of systemic spread of the silencing signal in the composite plants of both Arabidopsis and Medicago truncatula. It is shown that RNA silencing does not spread systemically to non-co-transformed (lateral) roots and only inefficiently to the non-transgenic shoot. Furthermore, evidence is presented which shows that RNAi is cell autonomous in the root epidermis.
♛Agrobacterium is a genus of Gram-negative bacteria established by H. J. Conn that uses horizontal gene transfer to cause tumors in plants.
♛ Agrobacterium tumefaciens is the most commonly studied species in this genus.
♛RNA interference (RNAi) is a biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules.
♛Cry1Ac protoxin is a crystal protein produced by the gram-positive bacterium, Bacillus thuringiensis during sporulation.
♛Meloidogyne incognita (root-knot nematode - RKN), also known as the "southern root-nematode" or "cotton root-knot nematode" is a plant-parasitic roundworm in the family Heteroderidae.
♛This nematode is one of the four commonest species worldwide and has numerous hosts. It typically incites large, usually irregular galls on roots as a result of parasitism.