Question

albert solution and its nutrient concentration

Answer 1

Using methods previously described, cultures of two clones of Marchantia polymorpha were supplied with ten nutrient solutions ranging in concentration from 1.83 to 0.01 atmospheres, of which solution 5, with a concentration of 0.21 atmospheres and 0.0033 mols per liter, was optimum for vegetative growth-as judged by fresh and dry weights and area. It contained 1.6 ccs. KNO3, 1.4 ccs. Ca(NO3)2, 1.2 cc. Mg(NO3)2, 0.8 cc. KH2PO4, and 1.6 ccs. MgSO4, each of a 0.5 M solution per liter, and the usual micro-nutrients. Solution 6 was by some standards slightly superior and by others inferior to solution 5. Its concentration was 75 percent that of solution 5. The pH characteristics of these solutions are briefly discussed. 2. Gemmae cups are most numerous on plants grown on solution 4. 3. Gemmae cups are produced on female and male plants in a ratio of 1 to 1.44-a lower ratio than in previous experiments. 4. Solutions with high salt concentration tended to kill the growing tips of plants and produced translucent thalli. Maturity was rapid, with cells slightly smaller than those grown on dilute solutions. Solutions low in salts favored the development of anthocyanin, numerous rhizoids, and a sturdy plant body possessing cells with living contents and thicker cell walls. 5. The calculation of dry weights of six plants from the weight of five and the fresh weights of six and five plants is reasonably accurate.